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KMID : 0363320060270030688
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Journal of Korean Oriental Internal Medicine
2006 Volume.27 No. 3 p.688 ~ p.697
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Effect of Trigonel/ae Semen on Peroxynitrite Scavenging Activity and Protein Expression of Proinflammatory Mediators
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Lee Cheol-Ho
Jung Ji-Chun
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Abstract
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Objectives : Peroxynitrite (), and nitric oxide (NO) are cytotoxic species that can oxidize several cellular components such as proteins. lipids and DNA. It has been implicated in the aging process and age-related disease such as Alzheimer’s disease, rheumatoid arthritis, cancer and atherosclerosis. The aim of this study was to investigate scavenging activities. and that of its precursors. NO and of Trigonellae Semen.
Methods : To investigate . NO. scavenging activities, fluorescent probes, namely 2’.7’-dichlorodihydrofluorescein diacetate (DCFDA). 4.5-diaminofluorescein (DAF-2) and dihydrorhodamine 123 (DHR 123) were used. Protein expression levels of iNOS, COX-2, NF-, and VCAM-1 were assayed by western blot.
Results : Trigonellae Semen markedly scavenged authentic , and NO. It also inhibited induced by and NO which are derived from SIN-1. Furthermore. Trigonellae Semen inhibited , and NO generation in LPS-treated ICR mouse kidney postmitochondria. Trigonellae Semen inhibited gene expression of iNOS, COX-2, VCAM-l and NF- (p65) activation.
Conclusions : These results suggest that Trigonellae Semen is an effective , and NO scavenger. and that this substance has a potential role as an inhibitor of the aging process, and in therapy against age-related diseases.
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KEYWORD
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Trigonellae Semen, peroxynitrite, nitric oxide, reactive oxygen species, iNOS, COX-2, VCAM-l
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